The isoprene-responsive phosphoproteome provides new insights into the putative signalling pathways and novel roles of isoprene

TitleThe isoprene-responsive phosphoproteome provides new insights into the putative signalling pathways and novel roles of isoprene
Publication TypeJournal Article
Year of Publication2023
AuthorsWeraduwage, SM, Whitten, D, Kulke, M, Sahu, A, Vermaas, JV, Sharkey, TD
JournalPlant, Cell & Environment
Date Published2023/12/01
ISBN Number0140-7791
Keywordsphosphorylation, plant growth regulator, signalling networks
Abstract

Many plants, especially trees, emit isoprene in a highly light- and temperature-dependent manner. The advantages for plants that emit, if any, have been difficult to determine. Direct effects on membranes have been disproven. New insights have been obtained by RNA sequencing, proteomic and metabolomic studies. We determined the responses of the phosphoproteome to exposure of Arabidopsis leaves to isoprene in the gas phase for either 1 or 5?h. Isoprene effects that were not apparent from RNA sequencing and other methods but were apparent in the phosphoproteome include effects on chloroplast movement proteins and membrane remodelling proteins. Several receptor kinases were found to have altered phosphorylation levels. To test whether potential isoprene receptors could be identified, we used molecular dynamics simulations to test for proteins that might have strong binding to isoprene and, therefore might act as receptors. Although many Arabidopsis proteins were found to have slightly higher binding affinities than a reference set of Homo sapiens proteins, no specific receptor kinase was found to have a very high binding affinity. The changes in chloroplast movement, photosynthesis capacity and so forth, found in this work, are consistent with isoprene responses being especially useful in the upper canopy of trees.

URLhttps://doi.org/10.1111/pce.14776
Short TitlePlant, Cell & Environment